T7

Part:BBa_K3172100:Design

Designed by: Juan-Martin Portilla   Group: iGEM19_FSU   (2019-10-01)


Modified T7 Promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Previous literature, as well as other T7 promoters, suggest that the +1 to +6 initiation region typically consist of purines. In order to not deviate from commonly used and naturally occurring T7 promoters, we added this additional guanine.


Source

None

References